Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes.
نویسندگان
چکیده
Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.
منابع مشابه
Detection of Shiga Toxin-Producing Strains of Escherichia coli (O157:H7) isolated from specimens of urinary and stool by Multiplex-PCR method
Background: Infection of shiga toxin-producing Strains of Escherichia coli has some other dangerous and deadly effects such as hemolytic uremic syndrome (HUS) in addition to diarrhea. Its diagnosis is difficult and there is no information on It's incidence in Iran. The goal of this study is to evaluate and detect shiga toxinproducing strains of E. coli isolated from urinary and stool specimens ...
متن کاملDetection of Shiga toxin-producing Escherichia coli (STEC) in faeces of healthy calves in Mashhad, Iran
The aim of this study was to identify virulent Shiga toxin-producing Escherichia coli (STEC) strains isolated from faecal samples of 100 clinically healthy calves. In the present study, a total of 100 Escherichia coli (E. coli) isolates from clinically healthy calves belonging to 6 different farms located in Khorasan Razavi province, Iran, were examined for presence of virulence genes character...
متن کاملDetection and Molecular Characterization of Sorbitol Negative Shiga Toxigenic Escherichia Coli in Chicken from Northwest of Iran
Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens primarily associated with the consumption of contaminated ground beef and are an important food safety concern worldwide. STEC has been found to produce a family of related cytotoxins known as Shiga toxins (Stxs). Shiga toxins have been classified into two major classes, Stx1 and Stx2. A single strains of STEC can produce St...
متن کاملDetection of verotoxin (Shiga-like toxin)-producing and eae harboring Escherichia coli in some wild captive and domestic Equidae and Canidae
The aim of this study was to investigate the prevalence of STEC and EPEC strains and E. coli O157 serogroup in some Equidae and Canidae. The fecal samples of 79 animals from 6 different species were evaluated for presence of these strains. All the Isolates were tested for virulence genes using multiplex-PCR. Non-sorbitol fermenting (NSF) Escherichia coli isolates and positive strains for virule...
متن کاملIdentification of shiga toxin producing Escherichia coli O157:H7 in raw cow milk samples from dairy farms in Mashhad using multiplex PCR assay
In this study 130 bulk tank milk samples which were delivered to the Pegah Pasturisation Factory inMashhad were collected randomly during the summer months. Samples were firstly enriched in modifiedtrypticase soy broth containing novobiocin, followed by plating onto sorbitol MacConkey agar supplementedwith cefixime and potassium tellurite for isolation of Escherichia coli O157:H7. Consequently ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 18 شماره
صفحات -
تاریخ انتشار 2011